Epidemiology of apple leaf spot

An Investigation of Apple Chlorotic Leaf Spot (ACLSV) and Tomato Ring Spot (ToRSV) in Some Iranian Pear Gardens

Background and Aims: In spite of a long tradition of fruit-tree growing in all provinces of Iran, information on pear (Pyrus communis) tree viruses is scant. In order to identify of the nature of the virus like symptoms and decline in pear trees, we carried out a survey for the occurrence of four important viruses. Materials and Methods: Leaf samples of pear showing virus like symptoms wer...

The RT-PCR identification and sequence analysis of Apple chlorotic leaf spot virus from apple cultivars in Jiaodong Peninsula, China

A set of specific primer pairs was utilized to detect Apple chlorotic leaf spot virus (ACLSV) from seven different apple cultivars in Jiaodong Peninsula via reverse transcription polymerase chain reaction (RT-PCR), and the sequence of ACLSV genome was analysed. The results indicate that: (1) High-purity total RNA could be successfully isolated using plant RNA rapid extraction kit. The ratios of...

Genome Sequences of Three Apple chlorotic leaf spot virus Isolates from Hawthorns in China

The genome sequences of Apple chlorotic leaf spot virus (ACLSV) isolates from three accessions of hawthorns (Crataegus pinnatifida) grown at Shenyang Agricultural University were determined using Illumina RNA-seq. To confirm the assembly data from the de novo sequencing, two ACLSV genomic sequences (SY01 and SY02) were sequenced using the Sanger method. The SY01 and SY02 sequences obtained with...

The Multifunctional Roles of Apple chlorotic leaf spot virus 50KP Movement Protein

Diverse plant virus families encode movement proteins (MPs) in their genomes. MPs are required for cell-to-cell movement of the virus in host plants and modify plasmodesmata in the cell wall, allowing cell-to-cell movement of virus particles or infectious transcripts. In recent years, much progress has been made in determining cell-to-cell movement functions of MPs from herbaceous plant viruses...

Molecular Characterization and Diagnostic Development for Apple chlorotic leaf spot virus